Creation of active
Read details In this paper, we recombine half-barrel-sized segments of two hyperthermophile endoglucanases (a cellulose and a beta-glucosidase) into ‘chimeras’ in different ways and show interesting, and diverse, effects of…
Arsenic and 17-b-estradiol bind
Read details The binding of SDS to proteins at millimolar concentrations of SDS under conditions of pH in which a protein is negatively charged leads to the formation of amyloid…
Probing protease sensitivity
Read details This work was performed by a collaborator’s lab. My contribution to it was only one of the core ideas behind the work and some of the bioinformatics-based exploration,…
Direct N-terminal sequencing 2015
Read details We have developed a method for aminopeptidase-based sequence determination of peptides by MS (e.g., ordinary MALDI-TOF mass spectrometry) instead of by MS-MS mass spectrometry. Briefly, the TET aminopeptidase…
Single cell-level detection 2015
Read details We are studying various aspects of the DNA-binding of the Escherichia coli proteins, HU-A and HU-B. In doing so, we have generated fluorescent protein-tagged versions of HU, using…
Structural stabilities and activities
Read details We show in this paper that triosephosphate isomerase (TIM) cloned and produced from two different hyperthermophile archaeal genomes catalyse the interconversion of glyceraldehyde-3-phosphate (G3P) and dihyroxyacetone phosphate (DHAP)…
Calcium Binding to Beta
Read details Beta-2-microglobulin (beta2m) is shed from MHCs and present in the serum which also contains calcium. We show that calcium binding to beta-2-microglobulin (beta2m) at physiological pH causes beta2m…